Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: The CXCL16 A181V Mutation Selectively Inhibits Monocyte Adhesion to CXCR6 but Is Not Associated With Human Coronary Heart Disease

doi: 10.1161/atvbaha.110.220558

Figure Lengend Snippet: Figure 1. SNPs within the CXCL16 gene. A, Cartoon of the CXCL16 protein. B, The genomic location, DNA sequence, and corresponding protein sequence resulting from nonsynonymous SNPs in CXCL16 as deduced from the HapMap database.19 C, The gene organization of CXCL16 on chromosome 17 (adapted from http://www.hapmap.org).

Article Snippet: Cell surface expression of L1.2 cells transiently transfected with HA-CXCR6 cDNA were detected using anti-HA and fluorescein isothiocyanate–conjugated goat anti-mouse IgG antibodies as previously described.16 Expression of CXCL16 on HEK293T cells was detected using biotinylated anti-human CXCL16 (R&D Systems) and streptavidin fluorescein isothiocyanate (Serotec Ltd, Oxford, United Kingdom) with goat IgG as negative control.

Techniques: Sequencing

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: The CXCL16 A181V Mutation Selectively Inhibits Monocyte Adhesion to CXCR6 but Is Not Associated With Human Coronary Heart Disease

doi: 10.1161/atvbaha.110.220558

Figure Lengend Snippet: Figure 2. Surface expression, shedding, and OxLDL uptake of WT and T123V181-CXCL16 transfectants. A, Cell surface expression of WT and T123V181-CXCL16. B, CXCL16 shed into the supernatant from WT and T123V181-CXCL16 transfectants. C, Migration of CXCR6 transfectants in response to WT and T123V181-CXCL16. D, OxLDL uptake in WT and I123T A181V-CXCL16 transfectants. *P0.05, **P0.01.

Article Snippet: Cell surface expression of L1.2 cells transiently transfected with HA-CXCR6 cDNA were detected using anti-HA and fluorescein isothiocyanate–conjugated goat anti-mouse IgG antibodies as previously described.16 Expression of CXCL16 on HEK293T cells was detected using biotinylated anti-human CXCL16 (R&D Systems) and streptavidin fluorescein isothiocyanate (Serotec Ltd, Oxford, United Kingdom) with goat IgG as negative control.

Techniques: Expressing, Migration

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: The CXCL16 A181V Mutation Selectively Inhibits Monocyte Adhesion to CXCR6 but Is Not Associated With Human Coronary Heart Disease

doi: 10.1161/atvbaha.110.220558

Figure Lengend Snippet: Figure 3. T123V181-CXCL16 does not mediate adhesion of CXCR6 L1.2 trans- fectants and is associated with the enhanced formation of multimers. A, Comparative adhesion of CXCR6 cells to transfectants expressing WT or T123V181-CXCL16. B, Western blot of lysates from WT or T123V181-CXCL16 transfectants. C, Relative CXCL16 cell surface expression of transfectants expressing WT, T123V181, T123, and V181 CXCL16. D, Comparative adhesion of CXCR6 cells to the same CXCL16 transfectants. *P0.05, **P0.01, ***P0.001.

Article Snippet: Cell surface expression of L1.2 cells transiently transfected with HA-CXCR6 cDNA were detected using anti-HA and fluorescein isothiocyanate–conjugated goat anti-mouse IgG antibodies as previously described.16 Expression of CXCL16 on HEK293T cells was detected using biotinylated anti-human CXCL16 (R&D Systems) and streptavidin fluorescein isothiocyanate (Serotec Ltd, Oxford, United Kingdom) with goat IgG as negative control.

Techniques: Expressing, Western Blot

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: The CXCL16 A181V Mutation Selectively Inhibits Monocyte Adhesion to CXCR6 but Is Not Associated With Human Coronary Heart Disease

doi: 10.1161/atvbaha.110.220558

Figure Lengend Snippet: Figure 4. T123V181 homozygosity is associated with a loss of CXCR6- mediated adhesion. A, Expression of WT CXCL16 on monocytes following culture with IFN- and TNF-. Filled and unfilled histograms represent control staining and CXCL16-specific staining, respec- tively. B and C, Cell surface and shed CXCL16 concentrations following culture of monocytes from donors. D, Adhesion of naı¨ve and CXCR6 L1.2 cells to monocytes from donors. *P0.05.

Article Snippet: Cell surface expression of L1.2 cells transiently transfected with HA-CXCR6 cDNA were detected using anti-HA and fluorescein isothiocyanate–conjugated goat anti-mouse IgG antibodies as previously described.16 Expression of CXCL16 on HEK293T cells was detected using biotinylated anti-human CXCL16 (R&D Systems) and streptavidin fluorescein isothiocyanate (Serotec Ltd, Oxford, United Kingdom) with goat IgG as negative control.

Techniques: Expressing, Control, Staining